Objectives: To present a handy survey of the myofascial classification of the muscles according to their pain region in function of their focus and radiation. With this survey the myofascial muscle chains can be consulted instantly. Methods: In function of the myofascial pain regions, per muscle, painfocus and radiation pain have been visually presented on the computer, based upon data out of the international myofascial literature. Out of these findings, for each myofascial region, primary (muscles that give focal pain) and secondary (muscles that give radiation pain) myofascial muscle chains were determined and charted. Results: 1. Classification of the muscles divided into 61 pain regions according to focality and radiation. 2. Classification of the associated chains in dysfunction according to pain region. 3. Classification of the development of myofascial muscle chains departing from a well-defined muscle. 4. Classification of the myofascial muscle chains where a well-defined muscle occurs in the muscle chain of another muscle. Conclusions: The examiner or therapist can easily trace the myofascial dysfunctions with the help of a compact reference book (practical workbook). The progress of pain can be explained by the development of the myofascial muscle chains and so there is a point of application to dissolve myofascial dysfunctions efficiently by means of these causal muscle chains.